Researchers have built bacteria to detect mutated DNA from cancer cells.

A team based at the University of Adelaide has engineered bacteria called Acinetobacter baylyi (A. baylyi), which is known for its ability to sample and integrate DNA from its environment. 

Researchers leveraged this natural competence to detect the mutated KRAS gene, an important driver of colorectal cancer.

The project was led by Dr Dan Worthley, Associate Professor Susan Woods and Dr Josephine Wright from SAHMRI and the University of Adelaide, in partnership with Professor Jeff Hasty at the University of California San Diego (UCSD).

A/Prof Woods says they dubbed this technology ‘CATCH’, which stands for ‘Cellular Assay of Targeted CRISPR-discriminated Horizontal gene transfer’.

“CATCH has the potential to detect bowel cancer early with the aim of preventing more people from dying of this and other cancers,” she said.

“This study demonstrates how bacteria can be designed to detect specific DNA sequences to diagnose disease in hard-to-reach places.”

In preclinical testing, the team found that the sensor bacteria were 100 per cent accurate in differentiating between models with and without colorectal cancer.

“We were thrilled to see transfer of DNA from the tumour to the sensor bacteria,” A/Prof Woods said.

“This shows that our biosensing system can be used to catch colorectal cancer DNA within a complex ecosystem.”

Dr Dan Worthley says potential applications of the technology extend beyond cancer.

“In the future we will detect and prevent many diseases, including bowel cancer, with cells, not drugs,” he said.

“We hope that this invention, of life detecting life, will be useful for clinicians, scientists and engineers to help the community wherever and whenever DNA detection is important.”

The full study is accessible here.